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Evaluation and Validation of Real-Time Reverse Transcription-PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus

We developed an assay for the detection and quantitation of norovirus with the LightCycler SYBR Green-based real-time reverse transcription-PCR (real-time LC RT-PCR) and previously published primers in the capsid and the polymerase gene. One hundred thirty-two stool specimens from the Provincial Lab...

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Main Authors: Pang, Xiaoli, Lee, Bonita, Chui, Linda, Preiksaitis, Jutta K., Monroe, Stephan S.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC522381/
https://ncbi.nlm.nih.gov/pubmed/15472327
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.10.4679-4685.2004
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