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Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform

A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensiv...

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Main Authors: Leblanc, Jason J., Pettipas, Janice, Davidson, Ross J., Tipples, Graham A., Hiebert, Joanne, Hatchette, Todd F.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2593261/
https://ncbi.nlm.nih.gov/pubmed/18945836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01446-08
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