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Family Shuffling of Expandase Genes To Enhance Substrate Specificity for Penicillin G

Deacetoxycephalosporin C synthase (expandase) from Streptomyces clavuligerus, encoded by cefE, is an important industrial enzyme for the production of 7-aminodeacetoxycephalosporanic acid from penicillin G. To improve the substrate specificity for penicillin G, eight cefE-homologous genes were direc...

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Vydáno v:Appl Environ Microbiol
Hlavní autoři: Hsu, Jyh-Shing, Yang, Yunn-Bor, Deng, Chan-Hui, Wei, Chia-Li, Liaw, Shwu-Huey, Tsai, Ying-Chieh
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC522083/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15466573/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.10.6257-6263.2004
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