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Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in living cells
Botulinum neurotoxins (BoNTs) act as zinc-dependent endopeptidases that cleave proteins required for neurotransmitter release. To detect toxin activity, fragments of the toxin substrate proteins, synaptobrevin (Syb) or synaptosome-associated protein of 25 kDa (SNAP-25), were used to link cyan fluore...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
2004
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC522023/ https://ncbi.nlm.nih.gov/pubmed/15465919 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404107101 |
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