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Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in living cells

Botulinum neurotoxins (BoNTs) act as zinc-dependent endopeptidases that cleave proteins required for neurotransmitter release. To detect toxin activity, fragments of the toxin substrate proteins, synaptobrevin (Syb) or synaptosome-associated protein of 25 kDa (SNAP-25), were used to link cyan fluore...

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Detalhes bibliográficos
Main Authors: Dong, Min, Tepp, William H., Johnson, Eric A., Chapman, Edwin R.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC522023/
https://ncbi.nlm.nih.gov/pubmed/15465919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404107101
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