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(15)N and (13)C-SOFAST-HMQC editing enhances 3D-NOESY sensitivity in highly deuterated, selectively [(1)H,(13)C]-labeled proteins

The ongoing NMR method development effort strives for high quality multidimensional data with reduced collection time. Here, we apply ‘SOFAST-HMQC’ to frequency editing in 3D HMQC-NOESY-HMQC and demonstrate the sensitivity benefits using highly deuterated and (15)N, methyl labeled samples in H(2)O....

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Détails bibliographiques
Publié dans:J Biomol NMR
Auteurs principaux: Rossi, Paolo, Xia, Youlin, Khanra, Nandish, Veglia, Gianluigi, Kalodimos, Charalampos G.
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5218894/
https://ncbi.nlm.nih.gov/pubmed/27878649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-016-0074-5
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