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Random shearing as an alternative to digestion for mitochondrial DNA processing in droplet digital PCR
Droplet digital PCR (ddPCR) is a quantitative assay that requires DNA fragmentation to maximize reaction efficiency. Here, we measured the proportion of mitochondrial DNA (mtDNA) carrying the “common deletion,” a rare event, to compare quantification sensitivities between alternative DNA fragmentati...
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| Veröffentlicht in: | Mitochondrion |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5218872/ https://ncbi.nlm.nih.gov/pubmed/27838478 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mito.2016.11.005 |
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