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Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR
The viscosity of genomic DNA can interfere with digital PCR systems that partition samples into oil droplets or microfluidic wells. Restriction digestion may reduce the viscosity, but the process is labor-intensive, and the buffer can alter the conditions for PCR. DNA fragmentation using the QIAshre...
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| Publicado no: | Biotechniques |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4948299/ https://ncbi.nlm.nih.gov/pubmed/24724845 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114159 |
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