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Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR

The viscosity of genomic DNA can interfere with digital PCR systems that partition samples into oil droplets or microfluidic wells. Restriction digestion may reduce the viscosity, but the process is labor-intensive, and the buffer can alter the conditions for PCR. DNA fragmentation using the QIAshre...

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Bibliografski detalji
Izdano u:Biotechniques
Glavni autori: Yukl, Steven A., Kaiser, Philipp, Kim, Peggy, Li, Peilin, Wong, Joseph K.
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4948299/
https://ncbi.nlm.nih.gov/pubmed/24724845
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114159
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