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Real‐time monitoring and control of the load phase of a protein A capture step

The load phase in preparative Protein A capture steps is commonly not controlled in real‐time. The load volume is generally based on an offline quantification of the monoclonal antibody (mAb) prior to loading and on a conservative column capacity determined by resin‐life time studies. While this res...

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Dettagli Bibliografici
Pubblicato in:Biotechnol Bioeng
Autori principali: Rüdt, Matthias, Brestrich, Nina, Rolinger, Laura, Hubbuch, Jürgen
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2016
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5215519/
https://ncbi.nlm.nih.gov/pubmed/27543789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26078
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