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RNA-Guided Cas9-Induced Mutagenesis in Tobacco Followed by Efficient Genetic Fixation in Doubled Haploid Plants

Customizable endonucleases are providing an effective tool for genome engineering. The resulting primary transgenic individuals (T(0)) are typically heterozygous and/or chimeric with respect to any mutations induced. To generate genetically fixed mutants, they are conventionally allowed to self-poll...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Schedel, Sindy, Pencs, Stefanie, Hensel, Götz, Müller, Andrea, Rutten, Twan, Kumlehn, Jochen
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5209389/
https://ncbi.nlm.nih.gov/pubmed/28101094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2016.01995
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