Display of Bacterial Lipase on the Escherichia coli Cell Surface by Using FadL as an Anchoring Motif and Use of the Enzyme in Enantioselective Biocatalysis

We have developed a novel cell surface display system by employing FadL as an anchoring motif, which is an outer membrane protein involved in long-chain fatty acid transport in Escherichia coli. A thermostable Bacillus sp. strain TG43 lipase (44.5 kDa) could be successfully displayed on the cell sur...

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Detalhes bibliográficos
Main Authors: Lee, Seung Hwan, Choi, Jong-Il, Park, Si Jae, Lee, Sang Yup, Park, Byoung Chul
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Physiology and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC520891/
https://ncbi.nlm.nih.gov/pubmed/15345384
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.9.5074-5080.2004
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