Cell Surface Display of Lipase in Pseudomonas putida KT2442 Using OprF as an Anchoring Motif and Its Biocatalytic Applications

We developed a new cell surface display system in Pseudomonas putida KT2442 using OprF, an outer membrane protein of Pseudomonas aeruginosa, as an anchoring motif in a C-terminal deletion-fusion strategy. The Pseudomonas fluorescens SIK W1 lipase gene was fused to two different C-terminal truncated...

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Autors principals: Lee, Seung Hwan, Lee, Sang Yup, Park, Byoung Chul
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317447/
https://ncbi.nlm.nih.gov/pubmed/16332850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8581-8586.2005
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