Cellular encoding of Cy dyes for single-molecule imaging

A general method is described for the site-specific genetic encoding of cyanine dyes as non-canonical amino acids (Cy-ncAAs) into proteins. The approach relies on an improved technique for nonsense suppression with in vitro misacylated orthogonal tRNA. The data show that Cy-ncAAs (based on Cy3 and C...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Leisle, Lilia, Chadda, Rahul, Lueck, John D, Infield, Daniel T, Galpin, Jason D, Krishnamani, Venkatramanan, Robertson, Janice L, Ahern, Christopher A
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2016
Assuntos:
Biochemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5207767/
https://ncbi.nlm.nih.gov/pubmed/27938668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.19088
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