Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries

BACKGROUND: We developed a method to make a various high quality random peptide libraries for evolutionary protein engineering based on a combinatorial DNA synthesis. RESULTS: A split synthesis in codon units was performed with mixtures of bases optimally designed by using a Genetic Algorithm progra...

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Bibliographic Details
Main Authors: Tabuchi, Ichiro, Soramoto, Sayaka, Ueno, Shingo, Husimi, Yuzuru
Format: Artigo
Language:Inglês
Published: BioMed Central 2004
Subjects:
Methodology Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC520752/
https://ncbi.nlm.nih.gov/pubmed/15341664
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-4-19
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