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Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries
BACKGROUND: We developed a method to make a various high quality random peptide libraries for evolutionary protein engineering based on a combinatorial DNA synthesis. RESULTS: A split synthesis in codon units was performed with mixtures of bases optimally designed by using a Genetic Algorithm progra...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC520752/ https://ncbi.nlm.nih.gov/pubmed/15341664 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-4-19 |
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