Benzoate- and Salicylate-Tolerant Strains of Escherichia coli K-12 Lose Antibiotic Resistance during Laboratory Evolution

Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic acid (up to 20 mM) buffered at external pH 6.5,...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Creamer, Kaitlin E., Ditmars, Frederick S., Basting, Preston J., Kunka, Karina S., Hamdallah, Issam N., Bush, Sean P., Scott, Zachary, He, Amanda, Penix, Stephanie R., Gonzales, Alexandra S., Eder, Elizabeth K., Camperchioli, Dominic W., Berndt, Adama, Clark, Michelle W., Rouhier, Kerry A., Slonczewski, Joan L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
Assuntos:
Physiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5203621/
https://ncbi.nlm.nih.gov/pubmed/27793830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02736-16
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