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Development of a qPCR Method to Measure Mitochondrial and Genomic DNA Damage with Application to Chemotherapy-Induced DNA Damage and Cryopreserved Cells

DNA damage quantitation assays such as the comet assay have focused on the measurement of total nuclear damage per cell. The adoption of PCR-based techniques to quantify DNA damage has enabled sequence- and organelle-specific assessment of DNA lesions. Here we report on an adaptation of a qPCR techn...

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Библиографические подробности
Опубликовано в: :Biology (Basel)
Главные авторы: Evans, Stephen O., Jameson, Michael B., Cursons, Ray T. M., Peters, Linda M., Bird, Steve, Jacobson, Gregory M.
Формат: Artigo
Язык:Inglês
Опубликовано: MDPI 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5192419/
https://ncbi.nlm.nih.gov/pubmed/27740596
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biology5040039
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