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Direct selection for sequences encoding proteases of known specificity.

We have developed a simple genetic selection that could be used to isolate eukaryotic cDNAs encoding proteases that cleave within a defined amino acid sequence. The selection was developed by using the transcription factor GAL4 from Saccharomyces cerevisiae as a selectable marker, a cloned protease...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Smith, T A, Kohorn, B D
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1991
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51831/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2052595/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.12.5159
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