Direct selection for sequences encoding proteases of known specificity.
We have developed a simple genetic selection that could be used to isolate eukaryotic cDNAs encoding proteases that cleave within a defined amino acid sequence. The selection was developed by using the transcription factor GAL4 from Saccharomyces cerevisiae as a selectable marker, a cloned protease...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1991
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51831/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2052595/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.12.5159 |
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