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Nonhomologous End-Joining Ligation Transfers DNA Regulatory Elements between Cointroduced Plasmids

Cointroduction of plasmids into mammalian cells is commonly used to investigate transcription factor regulation of reporter genes or to normalize transfection efficiency. We report here that cotransfected DNA molecules commonly transfer enhancer elements from one plasmid to another. Using separate R...

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Détails bibliographiques
Auteurs principaux: Ishikawa, Toshio, Lee, Eun Jig, Jameson, J. Larry
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2004
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC516743/
https://ncbi.nlm.nih.gov/pubmed/15367654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.24.19.8323-8331.2004
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