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Quantifying Kinase-specific Phosphorylation Stoichiometry Using Stable Isotope Labeling In a Reverse In-gel Kinase Assay
Despite recent advancements in large-scale phospho-proteomics, methods to quantify kinase-specific phosphorylation stoichiometry of protein substrates are lacking. We developed a method to quantify kinase-specific phosphorylation stoichiometry by combining the reverse in-gel kinase assay (RIKA) with...
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| Publicado no: | Anal Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5166985/ https://ncbi.nlm.nih.gov/pubmed/27808495 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b02599 |
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