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Polymerase chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme c of horseradish peroxidase.

The synthesis of a gene coding for horseradish peroxidase (HRP, isozyme c; EC 1.11.1.7) is described using a polymerase chain reaction (PCR)-mediated gene synthesis approach developed in our laboratory. In this approach, all the oligonucleotides making up the gene are ligated in a single step by usi...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Jayaraman, K, Fingar, S A, Shah, J, Fyles, J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1991
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51602/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1851991/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.10.4084
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