Cargando...

PCR-mediated direct gene disruption in Schizosaccharomyces pombe.

We have examined the feasibility and efficiency of PCR-mediated direct gene disruptions in the fission yeast Schizosaccharomyces pombe. In the present study, the S.pombe ura4+ gene was amplified by PCR with oligonucleotides that had short flanking regions ( approximately 40 bp) to the target gene. U...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Kaur, R, Ingavale, S S, Bachhawat, A K
Formato: Artigo
Lenguaje:Inglês
Publicado: 1997
Materias:
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC146527/
https://ncbi.nlm.nih.gov/pubmed/9023122
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!