PCR-mediated direct gene disruption in Schizosaccharomyces pombe.

We have examined the feasibility and efficiency of PCR-mediated direct gene disruptions in the fission yeast Schizosaccharomyces pombe. In the present study, the S.pombe ura4+ gene was amplified by PCR with oligonucleotides that had short flanking regions ( approximately 40 bp) to the target gene. U...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Kaur, R, Ingavale, S S, Bachhawat, A K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146527/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023122/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.5.1080
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