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PCR-mediated direct gene disruption in Schizosaccharomyces pombe.
We have examined the feasibility and efficiency of PCR-mediated direct gene disruptions in the fission yeast Schizosaccharomyces pombe. In the present study, the S.pombe ura4+ gene was amplified by PCR with oligonucleotides that had short flanking regions ( approximately 40 bp) to the target gene. U...
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Autores principales: | , , |
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Formato: | Artigo |
Lenguaje: | Inglês |
Publicado: |
1997
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Materias: | |
Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC146527/ https://ncbi.nlm.nih.gov/pubmed/9023122 |
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