PCR-mediated direct gene disruption in Schizosaccharomyces pombe.
We have examined the feasibility and efficiency of PCR-mediated direct gene disruptions in the fission yeast Schizosaccharomyces pombe. In the present study, the S.pombe ura4+ gene was amplified by PCR with oligonucleotides that had short flanking regions ( approximately 40 bp) to the target gene. U...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | Nucleic Acids Res |
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| Κύριοι συγγραφείς: | , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Oxford University Press
1997
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146527/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023122/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.5.1080 |
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