The T296V Mutant of Amorpha-4,11-diene Synthase is Defective in Allylic Diphosphate Isomerization but Retains the Ability to Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene

The T296V mutant of amorpha-4, 11-diene synthase catalyzes the abortive conversion of the natural substrate (E,E)-farnesyl diphosphate mainly into the acyclic product (E)-β-farnesene (88%) instead of the natural bicyclic sesquiterpene amorphadiene (7%). Incubation of the T296V mutant with (3R,6E)-ne...

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Publié dans:Biochemistry
Auteurs principaux: Li, Zhenqiu, Gao, Ruiping, Hao, Qinggang, Zhao, Huifang, Cheng, Longbin, He, Fang, Liu, Li, Liu, Xiuhua, Chou, Wayne K. W., Zhu, Huajie, Cane, David E.
Format: Artigo
Langue:Inglês
Publié: 2016
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5154624/
https://ncbi.nlm.nih.gov/pubmed/27933789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b01004
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