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The T296V Mutant of Amorpha-4,11-diene Synthase is Defective in Allylic Diphosphate Isomerization but Retains the Ability to Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene
The T296V mutant of amorpha-4, 11-diene synthase catalyzes the abortive conversion of the natural substrate (E,E)-farnesyl diphosphate mainly into the acyclic product (E)-β-farnesene (88%) instead of the natural bicyclic sesquiterpene amorphadiene (7%). Incubation of the T296V mutant with (3R,6E)-ne...
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| Publicat a: | Biochemistry |
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| Autors principals: | , , , , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5154624/ https://ncbi.nlm.nih.gov/pubmed/27933789 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b01004 |
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