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Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wide

The transcription cycle of RNA polymerase II (Pol II) correlates with changes to the phosphorylation state of its large subunit C-terminal domain (CTD). We recently developed Native Elongation Transcript sequencing using mammalian cells (mNET-seq), which generates single-nucleotide–resolution genome...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Nojima, Takayuki, Gomes, Tomás, Carmo-Fonseca, Maria, Proudfoot, Nicholas J
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5152764/
https://ncbi.nlm.nih.gov/pubmed/26844429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.012
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