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Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases
Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an anti-oxidant. Here, we use CRISPR–Cas9 to knockout RNH1 in HeLa cells. W...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5148631/ https://ncbi.nlm.nih.gov/pubmed/27806571 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b01003 |
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