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Biochemical Characterization of Laboratory Mutants of Extended-Spectrum β-Lactamase TEM-60

Three mutants of the extended-spectrum β-lactamase TEM-60, the P51L, K104E, and S164R mutants, were constructed by site-directed mutagenesis. The kinetic parameters of the mutated enzymes and interactions of inhibitors were significantly different from those of TEM-60, revealing that the L51P mutati...

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Detalhes bibliográficos
Main Authors: Caporale, Bibiana, Franceschini, Nicola, Perilli, Mariagrazia, Segatore, Bernardetta, Rossolini, Gian Maria, Amicosante, Gianfranco
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC514782/
https://ncbi.nlm.nih.gov/pubmed/15328132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.9.3579-3582.2004
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