LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation

Here we introduce LOVTRAP, an optogenetic approach for reversible, light-induced protein dissociation. LOVTRAP is based on protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in K(d). By reversibly sequestering proteins at mitochondria, w...

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Bibliographic Details
Published in:Nat Methods
Main Authors: Wang, Hui, Vilela, Marco, Winkler, Andreas, Tarnawski, Miroslaw, Schlichting, Ilme, Yumerefendi, Hayretin, Kuhlman, Brian, Liu, Rihe, Danuser, Gaudenz, Hahn, Klaus M
Format: Artigo
Language:Inglês
Published: 2016
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5137947/
https://ncbi.nlm.nih.gov/pubmed/27427858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3926
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