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LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation
Here we introduce LOVTRAP, an optogenetic approach for reversible, light-induced protein dissociation. LOVTRAP is based on protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in K(d). By reversibly sequestering proteins at mitochondria, w...
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| Publicado no: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5137947/ https://ncbi.nlm.nih.gov/pubmed/27427858 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3926 |
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