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The structure of a thermophilic kinase shapes fitness upon random circular permutation

Proteins can be engineered for synthetic biology through circular permutation, a sequence rearrangement where native protein termini become linked and new termini are created elsewhere through backbone fission. However, it remains challenging to anticipate a protein’s functional tolerance to circula...

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Detalles Bibliográficos
Publicado en:ACS Synth Biol
Autores principales: Jones, Alicia M., Mehta, Manan M., Thomas, Emily E., Atkinson, Joshua T., Segall-Shapiro, Thomas H., Liu, Shirley, Silberg, Jonathan J.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5122316/
https://ncbi.nlm.nih.gov/pubmed/26976658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.5b00305
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