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A transposase strategy for creating libraries of circularly permuted proteins
A simple approach for creating libraries of circularly permuted proteins is described that is called PERMutation Using Transposase Engineering (PERMUTE). In PERMUTE, the transposase MuA is used to randomly insert a minitransposon that can function as a protein expression vector into a plasmid that c...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2012
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3351165/ https://ncbi.nlm.nih.gov/pubmed/22319214 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks060 |
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