Assessment of configurations and chemistries of bridged nucleic acids-containing oligomers as external guide sequences: a methodology for inhibition of expression of antibiotic resistance genes

External guide sequences (EGSs) are short antisense oligoribonucleotides that elicit RNase P-mediated cleavage of a target mRNA, which results in inhibition of gene expression. EGS technology is used to inhibit expression of a wide variety of genes, a strategy that may lead to development of novel t...

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Publicat a:Biol Methods Protoc
Autors principals: Jackson, Alexis, Jani, Saumya, Davies-Sala, Carol, Soler-Bistué, Alfonso J C, Zorreguieta, Angeles, Tolmasky, Marcelo E
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2016
Matèries:
Methods Manuscript
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5108630/
https://ncbi.nlm.nih.gov/pubmed/27857983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpw001
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