CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002

Trans-acting regulators provide novel opportunities to study essential genes and regulate metabolic pathways. We have adapted the clustered regularly interspersed palindromic repeats (CRISPR) system from Streptococcus pyogenes to repress genes in trans in the cyanobacterium Synechococcus sp. strain...

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Pubblicato in:Metab Eng
Autori principali: Gordon, Gina C., Korosh, Travis C., Cameron, Jeffrey C., Markley, Andrew L., Begemann, Matthew B., Pfleger, Brian F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5107151/
https://ncbi.nlm.nih.gov/pubmed/27481676
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymben.2016.07.007
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