Transposon-facilitated DNA sequencing.

We describe here a transposon-based DNA sequencing strategy that allows the introduction of sequencing priming sites throughout a target sequence by bacterial mating. A miniplasmid was designed to select against transposon insertions into the vector. Sites of transposon insertion are mapped by the p...

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Main Authors: Strathmann, M, Hamilton, B A, Mayeda, C A, Simon, M I, Meyerowitz, E M, Palazzolo, M J
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC50994/
https://ncbi.nlm.nih.gov/pubmed/1847513
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