Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants.
Establishment of loss-of-function phenotypes is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTph1. Leaves are collected from batches...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , , , , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
1995
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41113/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7667260/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.18.8149 |
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