Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants.

Establishment of loss-of-function phenotypes is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTph1. Leaves are collected from batches...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Koes, R, Souer, E, van Houwelingen, A, Mur, L, Spelt, C, Quattrocchio, F, Wing, J, Oppedijk, B, Ahmed, S, Maes, T
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41113/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7667260/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.18.8149
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