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Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants.

Establishment of loss-of-function phenotypes is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTph1. Leaves are collected from batches...

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Autors principals: Koes, R, Souer, E, van Houwelingen, A, Mur, L, Spelt, C, Quattrocchio, F, Wing, J, Oppedijk, B, Ahmed, S, Maes, T
Format: Artigo
Idioma:Inglês
Publicat: 1995
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC41113/
https://ncbi.nlm.nih.gov/pubmed/7667260
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