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Probing Intracellular Element Concentration Changes during Neutrophil Extracellular Trap Formation Using Synchrotron Radiation Based X-Ray Fluorescence

High pressure frozen (HPF), cryo-substituted microtome sections of 2 μm thickness containing human neutrophils (white blood cells) were analyzed using synchrotron radiation based X-ray fluorescence (SR nano-XRF) at a spatial resolution of 50 nm. Besides neutrophils from a control culture, we also an...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: De Samber, Björn, Niemiec, Maria J., Laforce, Brecht, Garrevoet, Jan, Vergucht, Eva, De Rycke, Riet, Cloetens, Peter, Urban, Constantin F., Vincze, Laszlo
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5094720/
https://ncbi.nlm.nih.gov/pubmed/27812122
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0165604
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