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Specific initiation by RNA polymerase I in a whole-cell extract from yeast.

A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abun...

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Hlavní autoři: Schultz, M C, Choe, S Y, Reeder, R H
Médium: Artigo
Jazyk:Inglês
Vydáno: 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC50943/
https://ncbi.nlm.nih.gov/pubmed/1992452
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