Combined (13)C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

BACKGROUND: Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production. Sufficient supply of precursors, energy, and redox cofactors is crucial for high recombinant protein production. In our present work, we fo...

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Bibliografiske detaljer
Udgivet i:Bioresour Bioprocess
Main Authors: Liu, Ping, Huang, Mingzhi, Guo, Menglei, Qian, Jiangchao, Lin, Weilu, Chu, Ju, Zhuang, Yingping, Zhang, Siliang
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2016
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5093185/
https://ncbi.nlm.nih.gov/pubmed/27867835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40643-016-0124-6
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