Combined (13)C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

BACKGROUND: Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production. Sufficient supply of precursors, energy, and redox cofactors is crucial for high recombinant protein production. In our present work, we fo...

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Detalhes bibliográficos
Publicado no:Bioresour Bioprocess
Main Authors: Liu, Ping, Huang, Mingzhi, Guo, Menglei, Qian, Jiangchao, Lin, Weilu, Chu, Ju, Zhuang, Yingping, Zhang, Siliang
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2016
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5093185/
https://ncbi.nlm.nih.gov/pubmed/27867835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40643-016-0124-6
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