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Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

This protocol describes the generation of a three-dimensional (3D) ex vivo liver model and its application to the study and development of viral vector systems. The model is obtained by repopulating the extracellular matrix of a decellularized rat liver with a human hepatocyte cell line. The model p...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Hiller, Thomas, Röhrs, Viola, Dehne, Eva-Maria, Wagner, Anke, Fechner, Henry, Lauster, Roland, Kurreck, Jens
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092236/
https://ncbi.nlm.nih.gov/pubmed/27805597
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54633
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