siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Human primary villous cytotrophoblasts are a very useful source of primary cells to study placental functions and regulatory mechanisms, and to comprehend diseases related to pregnancy. In this protocol, human primary villous cytotrophoblasts freshly isolated from placentas through a standard DNase/...

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Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Lokossou, Adjimon Gatien, Toufaily, Chirine, Vargas, Amandine, Barbeau, Benoit
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2016
Konular:
Genetics
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092052/
https://ncbi.nlm.nih.gov/pubmed/27685614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53995
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