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siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Human primary villous cytotrophoblasts are a very useful source of primary cells to study placental functions and regulatory mechanisms, and to comprehend diseases related to pregnancy. In this protocol, human primary villous cytotrophoblasts freshly isolated from placentas through a standard DNase/...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Lokossou, Adjimon Gatien, Toufaily, Chirine, Vargas, Amandine, Barbeau, Benoit
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092052/
https://ncbi.nlm.nih.gov/pubmed/27685614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53995
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