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A Fluorescence-based Assay of Phospholipid Scramblase Activity

Scramblases translocate phospholipids across the membrane bilayer bidirectionally in an ATP-independent manner. The first scramblase to be identified and biochemically verified was opsin, the apoprotein of the photoreceptor rhodopsin. Rhodopsin is a G protein-coupled receptor localized in rod photor...

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Foilsithe in:J Vis Exp
Main Authors: Ploier, Birgit, Menon, Anant K.
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2016
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092049/
https://ncbi.nlm.nih.gov/pubmed/27684510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54635
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