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Identification of the critical residues responsible for differential reactivation of the triosephosphate isomerases of two trypanosomes

The reactivation of triosephosphate isomerase (TIM) from unfolded monomers induced by guanidine hydrochloride involves different amino acids of its sequence in different stages of protein refolding. We describe a systematic mutagenesis method to find critical residues for certain physico-chemical pr...

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Veröffentlicht in:Open Biol
Hauptverfasser: Rodríguez-Bolaños, Monica, Cabrera, Nallely, Perez-Montfort, Ruy
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Royal Society 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5090059/
https://ncbi.nlm.nih.gov/pubmed/27733588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsob.160161
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