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Impact of Three Illumina Library Construction Methods on GC Bias and HLA Genotype Calling

Next-generation sequencing (NGS) is increasingly recognized for its ability to overcome allele ambiguity and deliver high-resolution typing in the human leukocyte antigen (HLA) system. Using this technology, non-uniform read distribution can impede the reliability of variant detection, which renders...

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Detalhes bibliográficos
Publicado no:Hum Immunol
Main Authors: Lan, James H, Yin, Yuxin, Reed, Elaine F, Moua, Kevin, Thomas, Kimberly, Zhang, Qiuheng
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5089167/
https://ncbi.nlm.nih.gov/pubmed/25543015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.humimm.2014.12.016
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