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Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase
Initiation is a highly regulated, rate-limiting step in transcription. We used a series of approaches to examine the kinetics of RNA polymerase (RNAP) transcription initiation in greater detail. Quenched kinetics assays, in combination with gel-based assays, showed that RNAP exit kinetics from compl...
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| Опубликовано в: : | Proc Natl Acad Sci U S A |
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| Главные авторы: | , , , , , , , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
2016
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5087071/ https://ncbi.nlm.nih.gov/pubmed/27729537 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1605038113 |
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