Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase

Initiation is a highly regulated, rate-limiting step in transcription. We used a series of approaches to examine the kinetics of RNA polymerase (RNAP) transcription initiation in greater detail. Quenched kinetics assays, in combination with gel-based assays, showed that RNAP exit kinetics from compl...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Lerner, Eitan, Chung, SangYoon, Allen, Benjamin L., Wang, Shuang, Lee, Jookyung, Lu, Shijia W., Grimaud, Logan W., Ingargiola, Antonino, Michalet, Xavier, Alhadid, Yazan, Borukhov, Sergei, Strick, Terence R., Taatjes, Dylan J., Weiss, Shimon
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
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PNAS Plus
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5087071/
https://ncbi.nlm.nih.gov/pubmed/27729537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1605038113
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