Quantitative Tagless Copurification: A Method to Validate and Identify Protein-Protein Interactions

Identifying protein-protein interactions (PPIs) at an acceptable false discovery rate (FDR) is challenging. Previously we identified several hundred PPIs from affinity purification - mass spectrometry (AP-MS) data for the bacteria Escherichia coli and Desulfovibrio vulgaris. These two interactomes h...

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Detalhes bibliográficos
Publicado no:Mol Cell Proteomics
Principais autores: Shatsky, Maxim, Dong, Ming, Liu, Haichuan, Yang, Lee Lisheng, Choi, Megan, Singer, Mary E., Geller, Jil T., Fisher, Susan J., Hall, Steven C., Hazen, Terry C., Brenner, Steven E., Butland, Gareth, Jin, Jian, Witkowska, H. Ewa, Chandonia, John-Marc, Biggin, Mark D.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2016
Assuntos:
Technological Innovation and Resources
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5083090/
https://ncbi.nlm.nih.gov/pubmed/27099342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M115.057117
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